W. A. Gutierrez, H. D. Shew, and T. A. Melton
A semi-selective medium was developed to isolate
Rhizoctonia solani fron soil samples and infected tissue. This
medium allow a faster growth of R. solani colonies compared to the
standard water agar medium.
After isolation, dishes are incubated in the dark at 21 to 23 C. After 24 hours of incubation, dishes are evaluated to assess the number of R. solani colonies present.
Colonies of R. solani have a characteristic growth morphology and grow very fast on this selective medium. Colonies can be easily identify after 12 to 24 hour of incubation in the dark. The alkalinity of the medium allow a faster grow of R. solani colonies compared to other fungi, which are more or less inhibited. This medium is effective to isolate and to clean colonies of most of the anastomosis groups of Rhizoctonia solani.
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