Detection of Phytophthora in Greenhouse Water Supplies |
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At 2-3 different points in greenhouse. |
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Collect about 5 gals of water in opaque plastic storage bins (bins
should be cleaned with bleach and rinsed completely). |
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Water level should be about 4-5 inches in bin. |
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Float green, rock hard pears on water, about 5 pears/bin for 3 days. |
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After 3 days, remove pears from water. |
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Place each pear in a sandwich-size recloseable bag. |
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Place bagged pears from each water sample in a larger bag to keep
pears from the same bin together. |
In ponds supplying greenhouse
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Supplies: Inner tube, (3/16" or 1/4") poly rope, and citrus-type mesh
bag. |
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Place 5-7 pears in citrus bag. |
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Attach bag in center of inner tube with rope straps so that it is
free to settle into the water. |
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If turtles are in the pond construct a cage of wire mesh hardware
cloth to protect pears from being eaten. |
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Attach 100' poly rope on either side of inner tube at the corner of
the pond; place tube and bag in water; then have two people walk in
opposite directions away from corner to draw tube out into pond. |
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Secure rope to stakes on bank so that tube will remain fairly stationary. After 3 days, retrieve tubes, pack and ship pears. |
Pears
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Preparation:
Purchase pears directly from wholesalers such that the pears are coming
directly out of cold storage. It is important that green, rock-hard
pears be used. |
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Just before use: Wash
pears gently in warm, soapy, mild dish detergent to remove any fungicide
residues. Rinse
pears in clean water and blot dry with paper towels. |
Packing pears for shipment
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Use individual recloseable sandwich bags for each pear. |
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Put pears from each location in greenhouse or pond in a larger plastic
bag and mark location accordingly. |
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Pack in shipping container with plenty of padding to avoid bruising
fruit during shipment. |
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Ship for next day delivery. |
| Ship
to: Plant Disease & Insect Clinic 1227 Gardner Hall 100 Derieux Place NC State University Raleigh, NC 27695-7211 |
Fees for Pear Bait testing are the same as other samples. Current Fees Phone:
919.515.3619 |
Plan sampling so pears can be shipped Monday - Wednesday
Web page last updated February 2007 by T. Creswell